Immunology Services

ImQuest BioSciences, a preclinical CRO, provides cutting edge services for the assessment of products that modulate the immune response. With our decades of immunology experience we can utilize cell lines or primary cells to efficiently assess the effect of compound treatment on the expression of cytokines, cell proliferation and cell phenotype modulation in support of your project needs.

Contact us to learn more about our expertise in primary cell culture and in the design and implementation of custom in vitro assays.

Luminex Platform for Cytokine Evaluation

Using the state of the art Luminex xMAP Intelliflex, we can efficiently explore cytokine and chemokine expression from a wide variety of biological matrixes, from a range of host species in a multiplexing format. Up to 80 analytes can be assessed in a single assay well from readily accessible commercial kits, or you can design your own custom assay set. Explore a wide variety of ProcartaPlex Panels or custom panels that can be designed and assessed at ImQuest. Various Luminex panels by other manufacturers can be accommodated. Extremely small volume requirements and high sensitivity make the Luminex system uniquely suited for high throughput applications.

Learn more about the Luminex xMAP Intelliflex.

Explore readily accessible ThermoFisher Multiplexing kits.

Need a custom assay? Contact us to explore additional capabilties.

ELISpot, ELISA and Custom Immunoassay development

Leveraging our experience in immunoassay development implementation, ImQuest BioSciences can design and execute in vitro culturing and endpoint analysis for the assessment of immune functions T, B, NK and other mononuclear blood cells. Utilizing our CTL ImmunoSpot S6 Universal Analyzer, we can quickly design and implement a highly sensitive ELIspot or FluoroSpot reading. Our equipment can also be leveraged for any microtiter plate based assays requiring foci determination in a multi-color or single color format.

Readily available ELIspot and Flurospot kits can be utilized. Contact us to learn more.

We are also available to support the development of cell based assays with custom ELISA endpoint applications. With decades of experience, or team is unique posited to design, implement and analyze your custom immunoassay.

Multiparameter Flow Cytometry

A broad range of assay capabilities and endpoint can be accommodated utilized our flow cytometry experience. They include, but are not limited to:

  • Multi-parameter flow cytometry analysis up to 8 parameters using our FACSCantoII flow cytometer
  • Custom assay development and validation
  • Immunophenotyping
  • Cell Exhaustion and Activation Marker Analysis
  • Apoptosis and Necrosis Assays
  • Intracellular cytokine assays (ICS)
  • Cell Viability Assays
  • Proliferation assays in response to specific peptide antigens or mitogens (Con A, PHA, etc.)
  • Intracellular Calcium Flux Assays