HBV Antiviral Testing Services

ImQuest has developed a multi-parameter microtiter assay to expedite HBV antiviral testing to detect and analyze diverse markers of hepatitis B virus (HBV) replication and to gain insight into mechanism of action. The assay can be customized to meet Client development needs.

As a preclinical CRO, ImQuest BioSciences has extensive experience in antiviral testing service for the development of novel therapeutic and prevention products.

Innovative Screening Platform: HBV Antiviral Testing

ImQuest has developed a multi-parameter microtiter assay to expedite the screening of antiviral agents against multiple markers of hepatitis B virus (HBV) replication. The assay can be customized to meet your development needs and determine compound mechanism of action.

The platform has been optimized for standard cell lines (HepG2.2.15, Hep AD38, Hep AD79, and hNTCP) and primary human hepatocytes (PHHs).

Applicable for traditional small molecule therapeutics and biologics, modified for neutralization based assays and antibody based therapeutics, and genomic editing-based therapeutic applications.

Various qPCR and chemiluminescent immunoassay (CLIA) endpoints are available:

  • Extracellular HBV DNA
  • Intracellular HBV DNA
  • cccDNA
  • Pre-genomic RNA
  • Protein-based endpoints: HBsAg, HBeAg, HBcAg

Application include evaluation of range of action, time of addition assessments, efficacy of drug combinations, selection and characterization of resistant strains and serum binding effects on antiviral efficacy.

Primary Human Hepatocyte Screening

Infected PHHs can be used to screen for antiviral compounds targeting all stages of the HBV lifecycle. The PHH system developed at ImQuest more closely resembles natural HBV infection and is the gold standard for antiviral agent screening.

High Titer HBV Virus Stock Production

ImQuest BioSciences provides technical services for the production of high titer and highly infectious HBV virus stocks. We can propagate, concentrate, and characterize HBV at scales ranging from milliliters to liters, saving you time and avoiding the tedious steps required for HBV production. Whatever your needs, our scientists will customize the virus production process to your specifications.

Virus Production Services include:

  • Production of crude virus preparations from AD38, HepG2.2.15 and AD79 cell lines
  • Concentration of virus from crude preparations utilizing Tangential Flow Filtration (TFF)
  • Determination of virus concentration by qPCR and TCID50 determinations
  • Preparation of inactivated virus
  • Isolation of viral proteins, RNA & DNA
  • Quantification of proteins and nucleic acids

Documentation of final viral titer is provided with each lot of virus produced. Infectious virus in each pool may also be quantified by TCID50. Virus can be shipped domestically and internationally, and we are certified for the shipment of Category A and Category B Infectious Substances.

Preclinical Services Overview Click Here

Request a Scientific Consultation

We would love to hear more about your research and development needs. Contact us and we can coordinate a discussion with one of our technical directors to better understand you needs and develop a customized solution.

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Explore Additional Services

The ImQuest scientific team has extensive expertise in the development of small molecules, natural products, biologics and vaccines for the treatment and prevention of infectious disease, cancer, and inflammatory disease. Explore our additional service offering, or reach out to schedule a consultation.

Our Mission

Our goal is to help our clients accelerate their research and development efforts leading to the successful development of new products for the prevention and treatment of human disease. We have a very specific mission; accelerate your research and development plans and provide robust data in support of your projects.