Primary Immune Cell Assays

ImQuest BioSciences has decades of experience developing and validating primary immune cell assays for immune cell subsets, directly from primary blood and tissue samples.

Our services are available for the evaluation of host-targeting therapeutics and immune modulators, including small molecules, natural products, and biologics.

Custom assay design in primary immune cells is our specialty, from both human donors and a wide range of other animal species.  Assays can be utilized to define target efficacy, mechanism of action, and immune toxicity.  Contact us to learn more.

Technical Capabilities

Primary PBMC and T Cell Subsets

Sourcing and expertise in PBMC collection and analysis. Assay capabilities include, but are not limited to:

  • Custom primary PBMC bioassays
  • Collection and Fractionation of T Cell Subsets
  • Cell Proliferation Assays and Immuno-phenotyping
  • Mitogen and antigen stimulation assay
  • Cytokine production, stimulation and detection
  • Analysis of CD4+ and CD8+ Function
  • Infection models available (e.g. HIV)
  • Human, NHP, Rat, Canine, and Murine Cells

Macrophage and Dendritic Cells

Custom differentiation of macrophages and Dendritic Cells from primary PBMCs. Assay capabilities include, but are not limited to:

  • Isolation and Differentiation of macrophages and dendric cells
  • Custom Phenotype Generation (M1/M2, Mature and Immature DCs)
  • Primary mechanism, functional assays and in vitro toxicity assays
  • Human, NHP, Rat, Canine and Murine Cells
  • Analysis of phenotype and function
  • Phagocytosis assays
  • Viral and Bacterial Infection Models
  • Custom protein and gene expression endpoints

Analysis Technologies

Luminex Platform for Cytokine Evaluation

Using the Luminex xMAP Intelliflex, we can efficiently explore cytokine and chemokine expression from a wide variety of biological matrixes from a range of host species in a multiplexing format. Up to 80 analytes can be assessed in a single assay well from readily accessible commercial kits, or you can design your own custom assay set. Explore a wide variety of ProcartaPlex Panels or custom panels that can be designed and assessed at ImQuest. Various Luminex panels by other manufacturers can be accommodated. Extremely small volume requirements and high sensitivity make the Luminex system uniquely suited for high throughput applications.

Learn more about the Luminex xMAP Intelliflex.

Explore readily accessible ThermoFisher Multiplexing kits.

Biochemical Analysis

  • Immunoblot (western blot) design, optimization and analysis
  • Antibody selection and validation
  • Quantitative immunoblot analysis
  • Protein-protein interaction and immunoprecipitation assays
  • Protein electrophoresis: SDS-PAGE and native PAGE
  • Protein extraction from a wide variety of cell lines, primary cell, tissues and biological matrices
  • Cell fractionation and organelle isolation
  • Radiometric macromolecular synthesis evaluations
  • Explore More

ELISpot, ELISA and Custom Immunoassay Development

Leveraging our experience in immunoassay development implementation, ImQuest BioSciences can design and execute in vitro culturing and endpoint analysis for the assessment of immune functions T, B, NK and other mononuclear blood cells. Utilizing our CTL ImmunoSpot S6 Universal Analyzer, we can quickly design and implement a highly sensitive ELISpot or FluoroSpot reading. Our equipment can also be leveraged for any microtiter plate based assays requiring foci determination in a multi-color or single color format for primary immune cell assays.

Readily available ELIspot and Flurospot kits can be utilized. Contact us to learn more.

We are also available to support the development of cell-based assays with custom ELISA endpoint applications. Our team is uniquely positioned to design, implement and analyze your custom immunoassay.

Gene Expression Analysis

  • DNA, RNA extraction (total, intracellular, extracellular) from a wide variety of cell lines, primary cell, tissues and biological matrices
  • Plasmid DNA production
  • In vitro RNA transcription
  • Quantitative real time PCR Services
    • DNA and RNA quantification
    • Custom primer/probe development and verification
    • Gene expression analysis
    • Viral specific qPCR (RNA and DNA viruses)
  • Explore More

Request a Scientific Consultation

We would love to hear more about your research and development needs. Contact us and we can coordinate a discussion with one of our technical directors to better understand you needs and develop a customized solution.

Can’t find what you are looking for? Reach out to us, we can help.

Explore Additional Services

The ImQuest scientific team has extensive expertise in the development of small molecules, natural products, biologics and vaccines for the treatment and prevention of infectious disease, cancer, and inflammatory disease. Explore our additional service offering, or reach out to schedule a consultation.

Our Mission

Our goal is to help our clients accelerate their research and development efforts leading to the successful development of new products for the prevention and treatment of human disease. We have a very specific mission; accelerate your research and development plans and provide robust data in support of your projects.