Overview
ImQuest BioSciences offers full service, and customizable, research and development support for molecular biology applications. With state-of-the-art laboratories and decades of expertise, we provide a broad range of genomic and molecular based assay capabilities.
Our team is experienced with customized assay design, endpoint modification, and internalization and validation of pre-developed or published methods. Contact us to discuss assay needs not specifically listed.
Our capabilities include, but are not limited to the following:
Technical Capabilities
Nucleic Acid Services
- Technical services
- DNA, RNA extraction (total, intracellular, extracellular) from a wide variety of cell lines, primary cell, tissues and biological matrices
- Plasmid DNA production
- In vitro RNA transcription
- Quantitative real time PCR Services
- DNA and RNA quantification
- Custom primer/probe development and verification
- Gene expression analysis
- Viral specific qPCR (RNA and DNA viruses)
- Sequencing Services
- Gene specific sanger sequencing
- NGS Sequencing applications and support (RNASeq, Whole Genome Sequencing, ect.)
- Viral and bacterial sequencing and analysis
Molecular Cloning Services
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- DNA cloning services and consultation
- Custom plasmid generation and expression
- Restriction enzyme cloning strategies
- Specialized and project specific cloning strategies (Gateway, TOPO, Ect.)
- Site directed mutagenesis and gene synthesis support
Biochemical Analysis
- Protein Analysis
- Immunoblot (western blot) design, optimization and analysis
- Antibody selection and validation
- Quantitative immunoblot analysis
- Protein-protein interaction and immunoprecipitation assays
- Protein electrophoresis: SDS-PAGE and native PAGE
- Protein extraction from a wide variety of cell lines, primary cell, tissues and biological matrices
- Cell fractionation and organelle isolation
- Radiometric macromolecular synthesis evaluations
- Immunoassay and Biomarker Support
- Custom ELISA development and analysis
- Luminex evaluation of cytokines and chemokines (supernatant or serum, multiple species available)
- Custom biochemical assay support (commercially available reagents/kits, on request)
- Human DNA polymerase biochemical assays
- Mitochondrial functional assays: protein biogenesis and genomic material quantification
Cell-Based Molecular Assay Services
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- Cell line and primary cell based discovery research support
- Lentiviral vector design consultation and transduction services
- Gene silencing methodologies (siRNA, shRNA, CRISPR, Ect.)
- Transfection services
- DNA and RNA
- Transfection efficiency confirmation
- Cell line and primary cells
- Lipid and electroporation based assay designs
- Generation of stable cell lines
- Cell based reporter gene assays
- Microbial resistance selection and characterization
- Viral resistance selection and characterization
- Cell line, bacterial stock and virus stock production and genotypic/phenotypic characterization
Request a Scientific Consultation
We would love to hear more about your research and development needs. Contact us and we can coordinate a discussion with one of our technical directors to better understand you needs and develop a customized solution.
Can’t find what your are looking for? Reach out to us, we can help.
Explore Additional Services
The ImQuest scientific team has extensive expertise in the development of small molecules, natural products, biologics and vaccines for the treatment and prevention of infectious disease, cancer, and inflammatory disease. Explore our additional service offerings, or reach out to schedule a consultation.
Our Mission
Our goal is to help our clients accelerate their research and development efforts leading to the successful development of new products for the prevention and treatment of human disease. We have a very specific mission; accelerate your research and development plans and provide robust data in support of your projects.