Technical Services

Molecular BiologyTissue CultureFlow CytometryBiochemistryBiologicsImmunoassays

Our biological services are an integrated component of the ImQuestSUCCESS platform and are also available as stand-alone service components of our infectious disease, cancer, tissue culture, cell biology, molecular biology, biochemistry and immunology research and development programs. Our technical capabilities are available to support and supplement your laboratory operations and discovery initiatives.

Infectious Disease and Cancer Services

ImQuest BioSciences offers non-GLP and GLP compliant services to define the efficacy, safety, and pharmaceutical property of drug candidates and potential new biologics from initial discovery and screening to IND-enabling studies. For more information on our virology services, microbiology services and cancer specific services please visit our ImQuestSUCCESS services page.

Molecular Biology Laboratory Services

ImQuest BioSciences provides full service laboratories equipped with state-of-the-art instrumentation for both molecular cloning and gene expression analysis. Our expert staff has years of experience in the fields of molecular biology, biochemistry, genetics, virology and microbiology and are capable of handling both routine tasks and custom research and development projects in order to provide a broad range of core molecular biology services, including:

  • Virus production and genotypic characterization
  • Sequencing and identification of antimicrobial target genes
  • Quantitative real time PCR
  • Single Nucleotide Polymorphism (SNP) detection
  • Cloning and sequencing of recombinant plasmids
  • Gene expression analysis
  • Recombinant protein expression and purification
  • Protein-protein and protein-nucleic acid interactions
  • Microbial resistance characterization
  • Novel target identification and assay development
  • Analysis of pathogen-host interactions

Tissue Culture, Cellular Biology and Repository Services

Utilizing over 20 years of in vitro cell and tissue culture experience, ImQuest BioSciences provides technical services in the following areas:

  • Preparation and maintenance of cell lines or primary cell cultures
  • Organ cultures
  • Virus stock production
  • Bacteria stock production
  • Cell and virus repository
  • Baculovirus expression systems
  • Procaryotic expression systems
  • Immunofluorescent microscopy

Flow Cytometry and Immunology Services

  • Multicolor Flow Cytometry:
    • Multi-parameter flow cytometry analysis up to 8 parameters
    • Custom assay development and validation
    • Immunophenotyping
    • Cell Exhaustion and Activation Marker Analysis
    • Apoptosis and Necrosis Assays
    • Intracellular cytokine assays (ICS)
    • Cell Viability Assays
    • Proliferation assays in response to specific peptide antigens or mitogens (Con A, PHA, etc.)
    • Intracellular Calcium Flux Assays
  • Antigen-specific ELIspot assays
  • Tissue cross-reactivity assays and immunohistochemistry
  • ELISAs:
  • Neutralizing antibody and other cell based assays
  • Biomarker discovery and validation
  • Determination of gene expression patterns by real time qPCR
  • PBMC isolation and immunogenicity screening
  • Analysis of clinical samples to determine the immune response evoked by the treatment

Biochemistry and Enzymology Laboratory Services

ImQuest Biosciences' provide full service laboratories for biochemistry and enzymology as an integrated component of the ImQuestSUCCESS platform. Our laboratories are equipped with state-of-the-art instrumentation for analysis of enzyme activity and macromolecular interactions. ImQuest BioSciences' staff provides expertise in early to late-stage preclinical protein drug development from protein expression and optimization to purification protocol design through analytical and bioanalytical characterizations and formulation. With a fully outfitted core biochemistry and immunology laboratory facility, we are capable of providing services covering the following functions.

  • Protein production
  • Protein analytical and bioanalytical services
  • Protein therapeutic support
  • Enzymology
  • Standard and customized antiviral enzyme assays
  • Host cell polymerase assays
  • Assay development and optimization
  • Custom formulation and drug delivery services
  • Drug development support

Development of Biologic Products

ImQuest BioSciences understands that biologics require a set of specific methodologies to ensure the identity, strength, quality, purity, and potency of the product. We develop robust, high quality assays to measure these product attributes. Characterizing your product early in the development process can yield important information regarding manufacturing and process development, stability, and factors affecting the potency of the agent. All assays can be validated and GLP compliant as necessary. Learn More

Development of Immunoassays and Bioassays for Pharmacokinetic Evaluation and Development of Biologics

The development of biologic products for infectious disease, cancer or any other human health condition requires the development of GLP-compliant immuno- and bio-assays to quantify the pharmacokinetic profile of the subject product, understand the immunogenicity of the product when provided to animals or humans, and to understand if the immunogenicity results in neutralization of the product. ImQuest BioSciences has the capability and experience to develop, qualify, validate and perform GLP-compliant immunoassays to support the evaluation of biologics through animal safety/toxicology and human clinical trials. Learn More