Murine Model of Infection: Sepsis
Following initial in vitro screening to assess the efficacy, range and mechanism of antibacterial and antifungal activity and resistance, quickly transition your compound evaluation to immune-competent in vivo models. The sepsis mouse model is popular for its use of small and inexpensive animals, short duration experiments, and reproducible results for both clinical and microbiological assay endpoints. It has been used extensively in antimicrobial research, and because the animals are immune-competent, the model has increasingly been utilized for novel host-and immune-targeted products. ImQuest has optimized the sepsis model and it is available for testing both wild type and resistant bacterial strains based on the needs of our clients.
- Determination of in vivo efficacy of candidate antimicrobial drugs against clinical and antibiotic resistant strains
- Assessment of host directed products in an immune competent system
- Comparison of antimicrobial agents with FDA approved compounds of similar class
- Modulation of antibiotic time of addition
- Parallel assessment of PK/PD measurements, dose and dosing interval related to efficacy and the emergence of antibacterial resistance
Numerous wild type, resistant, and clinical strain are available for use in the mouse model. While the model has been optimized with a representative and client-specific strains, initial pilot studies to assess bacterial inoculum and growth kinetics is typically necessary. Client derived laboratory or clinical strains can be utilized in the model upon request. Variables in the model that can be customized include, route of drug administration, selection of positive control, duration of assay, animal dosing regimen, and assay endpoints assessed.
Contact us to learn more and let our microbiology expert help design and implement a study to fit your needs. Custom in vivo models are also available upon request.