ELISA Development, Optimization and Validation

ELISA Technical Services

ImQuest BioSciences has years of technical experience developing and performing ELISAs in support of our research services program. Our biological technical services are an integrated component of our ImQuestSUCCESS platform and are also available as stand-alone service components of our infectious disease, cancer, tissue culture, cell biology, molecular biology, biochemistry and immunology research and development programs.

Depending on your target of choice, ImQuest's technical team can help design and execute your ELISA methodology based on your custom cell based assay. Whether utilizing commercially available reagents or assisting in the development of a novel ELISA method, all ELISA assays are assessed for feasibility in consultation with our clients, developed and rigorously optimized. ImQuest has decades of tissue culture experience and can customize any cell based assay to meet our client needs. We have experience with both primary and cell line based systems.

Contact us to learn more about our ELISA based services.

GLP Immunoassay Development Services

With years of experience, ImQuest BioSciences can design, optimize, and validate your immunoassay in a GLP-compliant manner to support the evaluation of your new biologic – either biotherapeutic or vaccine – in preclinical and clinical development.

Our extensive experience with tissue culture and assay development is essential for the development of ELISA-based technology used to provide highly sensitive and robust detection of biomolecules in animals and humans.

ImQuest scientists develop a wide variety of immunoassays for use in support of toxicology and clinical trial programs. Immunoassays can be performed utilizing ELISA, ELIspot and flow cytometry, as well as other cell-based and bio-assays.

Immunoassay Development Methodology

ImQuest scientists conduct a feasibility study to replicate your assay, determine its robustness and sensitivity, and define required development steps. We then optimize the existing methodology to define the most appropriate, sensitive, and robust assay for validation. ImQuest scientists can also modify an assay to detect biologic vaccine product and sequentially perform immunogenicity and neutralization studies in three species as required. ImQuest’s team of scientists then analyzes your samples in a GLP-compliant manner and prepares a report.

Contact us to learn more about our ELISA and GLP-Immunoassay technical services.

ImQuest BioSciences is a preclinical contract research and development organization (CRO) that provides service to evaluate the potential efficacy of new and novel pharmaceutical products for the treatment and prevention of viruses, bacteria, cancer and inflammatory disease, including in vitro toxicology services.